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fluorescence resonance energy transfer

Fluorescence Resonance Energy Transfer

ISCID Encyclopedia of Science and Philosophy:\r\nFRET(Fluorescence Resonance Energy Transfer) is a technique for measuring interactions between two proteins in vivo. In this technique, two different fluorescent molecules (fluorophores) are genetically fused the two proteins of interest. Regular (non-FRET) fluorescence occurs when a fluorescent molecule (fluorophore) absorbs electromagnetic energy of one wavelength (the excitation frequency) and re-emits that energy at a different wavelength (the emission frequency)

Levels: Undergrad

Tags: fluorophore fluorescence resonance energy transfer

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