fluorescence
This web site displays a series of light and confocal micrographs illustrating a variety of subcellular structures and organelles. This provides a useful educational resource for people interested in cytology who do not have access to advanced imaging technologies or cell biological expertise.
Levels: Middle School
Tags:
microscopy
cytology
organelles
laser scanning confocal
fluorescence
phase contrast
differential interference contrast
This educational site offers background information on fluorescence as well as various applications in which it can be used.
Levels: Undergrad
Tags:
fluorophore
fluorescence
photobleaching
stokes shift
resonance energy transfer
This image gallery contains thumbnails of over 30 of the most common cell lines, labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques.
Levels: High School
Tags:
fluorophore
bioluminescence
fluorescence
Various thumbnails of fluorescence staining in mammalian and insect cells.
Levels: Middle School
Tags:
fluorescence
rhodamine b
Animations and simulations dealing with fluorescence microscopy
Levels: High School
Tags:
microscopy
fluorescence
simulations
GFP is a fluorescent protein isolated from coelenterates. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. The gene for GFP has been isolated and has become a useful tool for making chimeric proteins of GFP linked to other proteins where it functions as a fluorescent protein tag. As a noninvasive fluorescent marker in living cells, it allows for a wide range of applications where it may function as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Levels: High School
Tags:
fluorophore
bioluminescence
fluorescence
green fluorescence protein (gfp)
These pages illustrate aspects of fluorescence spectroscopy that are useful for elucidating structural and dynamic properties of proteins. Fluorescent techniques can provide parameters consistent with crystallographic results. The various environments of the fluorophores of a folded protein and the unique stereochemistry of the polypeptide chain affect the fluorophores in many ways which can be used to characterize and to follow changes in the folded conformation in solution. Fluorescence-based techniques can give information about a protein's conformation, binding sites, solvent interactions, degree of flexibility, internal motions, rotational diffusion coefficient, and many other physicochemical parameters.
Levels: High School
Tags:
fluorophore
bioluminescence
fluorescence
protein
green fluorescence protein (gfp)
Rockwell
Thursday, 01 February 2007
Weiss
Thursday, 08 February 2007
Researchers at Harvard have imaged neurons in mice with fluorescent tags and have also imaged viruses in this way to create images of neuron bundles and cell viruses.
Levels: Middle School, Public, High School, Undergrad, and Grad
Tags:
fluorescence
neurons
virus
genetically modified organisms
gmo
mouse
Mouse neurons in genetically modified mice express various combinations of fluorescent proteins to create a rainbow of fluorescent colors. These mice are used to trace neural pathways.
Levels: Middle School, Public, High School, Undergrad, and Grad
Tags:
fluorescence
gmo
mouse
neuron
neural pathways
Glow in the dark cats
Levels: K5, Middle School, Public, High School, Undergrad, and Grad
Tags:
fluorescence
cloning
Fluorescence Microscopy movies
Levels: High School, Undergrad, and Grad
Tags:
microscopy
fluorescence
fluorescence microscopy
Regular course
By: Dr. Molinaro and Dr. Shackelord. This course targets freshmen students that are part of the Integrated Studies Program. The course is a basic interdiscip...
Description: There are things that you cannot see because your eyes only detect the light that comes from objects in the specific range of colors that are part of visible light. However there are some materials in our world are fluorescent. When you shine a black light upon them then they will glow back a bright color that was not there before.
Attribution:
Type: video/quicktime
Tags:
fluorescence
biophotonics
cells
money
teacher resource

This work is licensed under a
Creative Commons Attribution 3.0 United States License.
Uploaded over 3 years ago by chris.sumerlin
Description: This movie shows C.elegans moving under white light and then a green filter is inserted and the white light reduced and an ultraviolet light turned on to see the fluorescence of these genetically modified C.elegans with labeled nuclei of the nervous system.
Attribution:
Type: video/quicktime
Tags:
c.elegans
fluorescence
microscopy

This work is licensed under a
Creative Commons Attribution-Noncommercial-Share Alike 3.0 United States License.
Uploaded about 4 years ago by marcom
This site has the fluorescence spectra for many common foods including liquids and solids. Samples include olive oil, juices, beer, wine, sugar, beef, chocolate, pears, tomatoes, carrots, etc.
Levels: Undergrad, Grad, High School, Professional, and Public
Tags:
fluorescence
autofluorescence
food
A whole range of genetically modified organisms is shown including cats, dogs, monkeys, fish, and many more.
Levels: K5, Undergrad, Grad, Middle School, High School, Professional, and Public
Tags:
fluorescence
autofluorescence
Dublin Institute of Technology
fluorescence
updated 10/13/10
joined 10/13/10
Description: Biophotonics is an exciting field that utilizes light to image, detect and cure disease plus help us learn about basic biological processes. The field is possible because of the way in which different forms of light interact differently with living tissues. Instruments like pulse oxymeters can shine light through your finger and figure out how oxygenated your blood is! Other parts of the electromagnetic spectrum can tell you different types of information. Fluorescence is also a very popular technique for labeling components of cells to better understand how they function. This handout helps explain the basic concepts in straightforward language.
Attribution: Marco Molinaro 2007
Type: application/pdf
Tags:
handout
teacher resource
biophotonics
near infrared
pulse oxymetry
fluorescence
poster

This work is licensed under a
Creative Commons Attribution-Noncommercial-Share Alike 3.0 United States License.
Uploaded over 1 year ago by marcom
Super resolution fluorescence microscopy was Nature Methods' choice for Method of the Year in 2008.
Levels: Undergrad, Grad, and Professional
Tags:
microscopy
fluorescence