Researcher and Professor
trhuser@ucdavis.edu
Research in my group centers prominently around ultra-sensitive optical microscopy and spectroscopy. Research projects scale from fundamental molecular and biomolecular systems to individual cells and organisms. Specifically, projects include the nondestructive characterization of individual stem cells, cancer cells, and virus-infected cells. A major component is devoted to the development and application of novel super-resolution optical microscopy techniques to dissect virological synapses between cells and to determine the structure and dynamics of tunneling nanotubes between cells. My group also develops nanosensors and novel markers for the long-term cellular and molecular imaging of chemicals, small molecules, and macromolecules in individual cells; Raman and and advanced forms of nonlinear label-free imaging (coherent anti-Stokes Raman spectroscopy (CARS) , doubly-resonant four-wave mixing (DR-FWM)) of individual cells, organelles, and lipoproteins, the study of DNA-protein, protein-protein, and protein-lipid interactions by single molecule fluorescence spectroscopy, and advanced live cell imaging techniques.
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Academic distinctions, awards
1998 Graduated Summa Cum Laude, University of Basel, Switzerland
2002 Norbert J. Kreidl Award of the Glass and Optical Division of the American Ceramic Society
2004 Excellence in Publication Award of the Associate Director, Chemistry and Materials Science, Lawrence Livermore National Laboratory
2006 “Above and Beyond” Award for undergraduate teaching, Center for Biophotonics
2008 - 2011 Visiting Professor, Department of Physics, University of Tromsø, Norway
2009 Guest Editor for Journal of Biophotonics on “Biophotonics in Regenerative Medicine”
Selected publications
2010 T. Weeks, I. Schie, S. Wachsmann-Hogiu, and T. Huser, “Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions” J. Biophot., DOI:10.1002/jbio. 10068, in print (2010)
2009 D.K. Lieu, J. Liu, C.W. Siu, G.P. McNerney, A. Abu-Khalil, T. Huser, R.A. Li, “Absence of Transverse Tubules Contributes to Non-Uniform Ca2+ Wavefront in Mouse and Human Embryonic Stem Cell-Derived Cardiomyocytes”, Stem Cells Dev. 18(10), 1493 - 1500 (2009)
2009 T. Weeks, S. Wachsmann-Hogiu, and T. Huser, “Raman Microscopy based on Doubly-Resonant Four-Wave Mixing (DR-FWM)”, Opt. Express 17(19), 17044- 17051 (2009)
2009 T. Nyunt, C. Dicus, Y.-Y. Cui, M. Yappert, T. Huser, M. Nantz, and J. Wu, "Physico-Chemical Characterization of Polylipid Nanoparticles for Gene Delivery to the Liver", Bioconjugate Chem. 20 (11), 2047-2054 (2009)
2009 T. Huser, C.A. Orme, C.W. Hollars, M.H. Corzett, and R. Balhorn, “Raman Spectroscopy of DNA Packaging in Individual Human Sperm Cells distinguishes Normal from Abnormal Cells”, J. Biophot. 2(5), 322 – 332 (2009) – Editor’s Choice Article
2009 W. Hübner, G.P. McNerney, B.M. Dale, P. Chen, R.E. Gordon, F.Y.S. Chuang, X.-D. Li, D.M. Asmuth, T. Huser, and B.K. Chen, “Quantitative 3D Video Microscopy of HIV Transfer Across T cell Virological Synapses”, Science 323, 1743-1747 (2009)
2009 S. Wachsmann-Hogiu, T. Weeks, and T. Huser, “Chemical analysis in vivo and in vitro by Raman spectroscopy – from single cells to humans”, Curr. Opin. Biotechnol. 20, 63-73 (2009) - Invited Review
2009 J. Chan, D. Lieu, T. Huser, and R. Li, “Label-free spectroscopic separation of human embryonic stem cells (hESCs) and their cardiac derivatives using Raman spectroscopy”Anal. Chem. 81, 1324–1331
2009 T. Laurence, G. Braun, C. Talley, A. Schwartzberg, M. Moskovits, N. Reich, and T. Huser, "Rapid, solution-based characterization of optimized SERS nanoparticle substrates", J. Am. Chem. Soc. 131(1), 162-169 (2009)
2008 T. Huser, “Nano-Biophotonics: new tools for chemical nano-analytics”, Curr. Opin. Chem. Biol. 12, 497–504 (2008) – Invited Review
2008 J.W. Chan, S. Fore, S. Wachsmann-Hogiu, and T. Huser, “Raman spectroscopy and microscopy of individual cells and cellular components”, Laser & Photon. Rev. 2(5), 325–349 (2008) – Invited Review, Cover Article
2008 N. Argov, S. Wachsmann-Hogiu, S.L. Freeman, T. Huser, C.B. Lebrilla, and J.B. German, "Size-Dependent Lipid Content in Human Milk Fat Globules Characterized by Laser Trap Raman Spectroscopy", J. Agric. Food Chem. 56(16), 7446-7450 (2008)
2008 J.W. Chan, D.S. Taylor, S. Lane, T. Zwerdling, J. Tuscano, and T. Huser, “Non-destructive Identification of Individual Leukemia Cells by Laser Tweezers Raman Spectroscopy”, Anal. Chem. 80, 2180-2187 (2008)
2008 B.S. Kim, C.C.I. Lee, J.E. Christensen, T.R. Huser, J.W. Chan, and A.F. Tarantal, “Growth, Differentiation, and Biochemical Signatures of Rhesus Monkey Mesenchymal Stem Cells”, Stem Cells Dev. 17, 185-198 (2008)
2008 I.W. Schie, T. Weeks, G.P. McNerney, S. Fore, J.K. Sampson, S. Wachsmann-Hogiu, J.C. Rutledge, and T. Huser, “Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting”, Opt. Express 16(3), 2168-2175 (2008)
2007 S. Ly, G. McNerney, S. Fore, J. Chan, and T. Huser, “Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence”, Opt. Express 15(25), 16839-16851 (2007)
2007 S. Fore, T.A. Laurence, C.W. Hollars, T. Huser, “Counting constituents in molecular complexes by fluorescence photon antibunching”, IEEE J. Select. Top. Quantum Electron. 13(4), 996-1005 (2007) – Invited paper
2007 S. Fore, Y. Yuen, L. Hesselink, T. Huser , “Pulsed-interleaved excitation FRET measurements on single duplex DNA molecules inside C-shaped nano-apertures”, Nano Letters 7(6), 1749-1756 (2007)
2007 A.M. Smith, T. Huser, and A.N. Parikh, “Dynamic recompartmentalization of supported lipid bilayers using focused femtosecond laser pulses”, J. Am. Chem. Soc. 129(9), 2422-2423 (2007)
2007 J.W. Chan, H. Winhold, M.H. Corzett, J.M. Ulloa, M. Cosman, R. Balhorn, and T. Huser “Monitoring Dynamic Protein Expression in Living E. Coli. Bacterial Cells by Laser Tweezers Raman Spectroscopy”, Cytometry A 71A, 468-474 (2007)
2006 S.O. Kucheyev, J.R. Hayes, J. Biener, T. Huser, C.E. Talley, and A.V. Hamza“Surface-enhanced Raman scattering on nanoporous gold”, Appl. Phys. Lett. 89, 053102 (2006)
2006 C.W. Hollars, J. Puls, O. Bakajin, B. Olsan, C.E. Talley, S.M. Lane, and T. Huser “Bio-assay based on single molecule fluorescence detection in microfluidic channels”, Anal. Bioanal. Chem. 385(8), 1384-1388 (2006)
2006 A.E. Miller, A.J. Fischer, T. Laurence, C.W. Hollars, R. Saykally, J.C. Lagarias, and T. Huser“Single molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy”, Proc. Nat. Acad. Sci. USA 103(30), 11136-11141 (2006)
2006 A.M. Schwartzberg, T. Oshiro, J.Z. Zhang, T. Huser, and C.E. Talley, “Improving nanoprobes using surface-enhanced Raman scattering from 30 nm hollow gold particles”, Anal. Chem. 78, 4732-4736 (2006)2006 T.A. Laurence, S. Fore, and T. Huser, “Fast, flexible algorithm for calculating photon correlations”, Opt. Lett. 31(6), 829-831 (2006)
2006 J.W. Chan, D. Taylor, T. Zwerdling, S.M Lane, K. Ihara, and T. Huser, “Micro-Raman Spectroscopy Detects Individual Neoplastic and Normal Hematopoietic Cells”, Biophys. J. 90(2), 648-656 (2006)
2005 S. Fore, T. Laurence, Y. Yeh, R. Balhorn, C.W. Hollars, M. Cosman, and T. Huser, “Photon correlation spectroscopy of discrete numbers of dye-molecules conjugated to DNA”,IEEE J. Select. Top. Quantum Electron. 11(4), 873-880 (2005)
2005 J.W. Chan, H. Winhold, S.M. Lane, and T. Huser, “Optical Trapping and Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy of Submicron-Sized Particles”, IEEE J. Select. Top. Quantum Electron. 11(4), 858-863 (2005)
2005 J.W. Chan, D. Motton, J.C. Rutledge, N. L. Keim, and T. Huser, “Raman Spectroscopic Analysis of Biochemical Changes in Individual Triglyceride-Rich Lipoproteins in the Pre- and Postprandial State”, Anal. Chem. 77, 5870-5876 (2005)This article was highlighted in “Research Profiles” in the October 1, 2005 issue of “Analytical Chemistry”, and “Spectroscopy Now”, November 2005
2005 C.E. Talley, J.B. Jackson, C. Oubre, N.K. Grady, C.W. Hollars, S.M. Lane, T. Huser, P. Nordlander, and N.J. Halas, “Surface-enhanced Raman Scattering from Individual Au Nanoparticles and Nanoparticle Dimer Substrates”, Nano Letters 5(8), 1569-1574 (2004)
2004 C.E. Talley, L. Jusinski, C.W. Hollars, S.M. Lane, and T. Huser, “Intracellular pH Sensors Based on Surface-Enhanced Raman Scattering”, Anal. Chem. 76, 7064-7068 (2004)
2004 A.M. Schwartzberg, C.D. Grant, A. Wolcott, C. Talley, T. Huser, R. Bogomolni, J.Z. Zhang“Unique Gold Nanoparticle Aggregates as a Highly Active SERS Substrate”,J. Phys. Chem. B 108, 19191 – 19197 (2004)
2004 J.K. Holt, A. Noy, T. Huser, D. Eaglesham, and Olgica Bakajin, “Fabrication of a Carbon Nanotube-Embedded Silicon Nitride Membrane for Studies of Nanometer-Scale Mass Transport”, Nano Letters 4, 2245-2250 (2004)
2004 J. Chan, A. P. Esposito, C. Talley, C. Hollars, S. Lane, and T. Huser, “Reagentless Identification of Single Bacterial Spores in Aqueous Solution by Confocal Laser Tweezers Raman Spectroscopy”, Anal. Chem. 76, 599-603 (2004)This article was highlighted in the April 2004 issue of “Biophotonics International”
2004 T. Huser, C.W. Hollars, W. Siekhaus, J.J. DeYoreo, T. Suratwala, T. Land, “Characterization of proton exchange layer profiles in KD2PO4 crystals by micro-Raman spectroscopy”, Appl. Spectrosc. 58, 349-351 (2004)
2003 A. P. Esposito, C. E. Talley, T. Huser, C. W. Hollars, C. M., Schaldach, and S. M. Lane, “Analysis of Single Bacterial Spores by Micro-Raman Spectroscopy”, Appl Spectrosc 57, 868-871 (2003)
2003 J.W. Chan, T. Huser, S.H. Risbud, J.S. Hayden, and D. M. Krol, “Waveguide fabrication in phosphate glasses using femtosecond laser pulses”, Appl Phys Lett 82, 2371-2373 (2003)
2003 C.W. Hollars, S.M. Lane, and T. Huser, “Controlled non-classical photon emission from single conjugated polymer molecules”, Chem Phys Lett 370, 393-398 (2003)
2003 A. Noy, and T. Huser, “Combined force and photonic probe microscope with single molecule sensitivity”, Rev Sci Inst 74, 1217-1221 (2003)This article was highlighted in the May 2003 issue of “Biophotonics International”
2003 J.W. Chan,, T.R. Huser, S.H. Risbud, and D.M. Krol, “Modification of the fused silica glass network associated with waveguide fabrication using femtosecond laser pulses”, Appl Phys A 76, 367-372 (2003)
2002 J. W. Chan, T. Huser, J. S. Hayden, S. H. Risbud, and D. M. Krol, “Fluorescence spectroscopy of color centers generated in phosphate glasses after exposure to femtosecond laser pulses”, J Am Ceram Soc 85, 1037-40 (2002)
2001 J. W. Chan, T. Huser, S. Risbud and D. M. Krol, “Structural changes in fused silica after exposure to focused femtosecond laser pulses”, Optics Letters 26, 1726-28 (2001)This article was highlighted in the April 2002 issue of "Laser Focus World"
2001 T. Huser and M. Yan, “Solvent-related conformational changes and aggregation of conjugated polymers studied by single molecule fluorescence spectroscopy”, J. Photochem. Photobiol. A 144, 43-51 (2001)
2001 F. I. Baida, D. Van Labeke, A. Bouhelier, T. Huser and D. W. Pohl, “Propagation and diffraction of locally excited surface plasmons”, J. Opt. Soc. Am. A 18, 1552-61 (2001)
2001 A. Bouhelier, T. Huser, H. Tamaru, H. J. Güntherodt, D. W. Pohl, F. I. Baida and D. Van Labeke, “Plasmon optics of structured silver films”, Phys. Rev. B 63, 155404-1 -155404-9 (2001)
2001 T. Huser and M. Yan, “Aggregation quenching in thin films of MEH-PPV studied by near-field scanning optical microscopy and spectroscopy”, Synth. Metals 116, 333-337 (2001)
2000 T. Huser, M. Yan and L. J. Rothberg, “Single chain spectroscopy of conformational dependence of conjugated polymer photophysics”, Proc. Nat. Acad. Sci. USA 97, 11187-11191 (2000)
1999 L. M. Eng, M. Bammerlin, C. Loppacher, M. Guggisberg, R. Bennewitz, R. Lüthi, E. Meyer, T. Huser, H. Heinzelmann and H. J. Güntherodt, “Ferroelectric domain characterisation and manipulation : a challenge for scanning probe microscopy”, Ferroelectrics 222, 411-420 (1999)
1999 H. Heinzelmann, J. M. Freyland, R. Eckert, T. Huser, G. Schürmann, W. Noell, U. Staufer and N. F. De Rooij, “Towards better scanning near-field optical microscopy probes - progress and new developments”, J Microsc 194, 365-368 (1999)
1999 A. Bouhelier, T. Huser, J. M. Freyland, H. J. Güntherodt and D. W. Pohl, “Plasmon transmissivity and reflectivity of narrow grooves in a silver film”, J Microsc 194, 571-573 (1999)
1999 T. Huser, L. Novotny, T. Lacoste, R. Eckert and H. Heinzelmann, “Observation and analysis of near-field optical diffraction”, J Opt Soc Am A 16, 141-148 (1999)
1998 T. Lacoste, T. Huser, R. Prioli and H. Heinzelmann, “Contrast enhancement using polarization-modulation scanning near-field optical microscopy (PM-SNOM) ”, Ultramicroscopy 71, 333-340 (1998)
1998 T. Huser, T. Lacoste, H. Heinzelmann and H. S. Kitzerow, “Scanning near-field optical microscopy of cholesteric liquid crystals”, J Chem Phys 108, 7876-7880 (1998)
1997 D. W. Pohl, H. Bach, M. A. Bopp, V. Deckert, P. Descouts, R. Eckert, H. J. Güntherodt, C. Hafner, B. Hecht, H. Heinzelmann, T. Huser, M. Jobin, U. Keller, T. Lacoste, P. Lambelet, F. Marquis-Weible, O. J. F. Martin, A. J. Meixner, B. Nechay, L. Novotny, M. Pfeiffer, C. Philipona, T. Plakhotnik, A. Renn, A. Sayah, J. M. Segura, B. Sick, U. Siegner, G. Tarrach, R. Vahldieck, U. P. Wild, D. Zeisel and R. Zenobi, “Optical microscopy in the nano-world”, Chimia 51, 760-767 (1997)
1997 T. Lacoste, T. Huser and H. Heinzelmann.,“Faraday-rotation imaging by near-field optical microscopy”, Z Phys B-Condens Matter 104, 183-184 (1997)
1996 H. Heinzelmann, T. Lacoste, T. Huser, H. J. Güntherodt, B. Hecht and D. W. Pohl.,“Instrumental Developments and Recent Experiments in Near-Field Optical Microscopy”, Thin Solid Films 273, 149-153 (1996)
1995 W. Gutmannsbauer, T. Huser, T. Lacoste, H. Heinzelmann and H. J. Güntherodt, “Scanning near-Field Optical Microscopy (SNOM) and Its Application in Mineralogy”, Schweiz Mineral Petrogr Mitt 75, 259-264 (1995)
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