The Fluorophore of Green Fluorescent Protein (GFP)

GFP is a fluorescent protein isolated from coelenterates. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. The gene for GFP has been isolated and has become a useful tool for making chimeric proteins of GFP linked to other proteins where it functions as a fluorescent protein tag. As a noninvasive fluorescent marker in living cells, it allows for a wide range of applications where it may function as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Levels: hs

Tags: fluorophore bioluminescence fluorescence green fluorescence protein (gfp)

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